This new and improved TAS saves about 17 seconds over my original TAS by taking a game over in level 4 and by using the fast kill strategy on the level 6 boss (which save about 4.5 and 13 seconds respectively).
#51216114062131000 - Battletoads & Double Dragon: The Ultimate Team (Truncated)
In the published run, there is a sequence of unnecessary inputs after the last necessary hit on the final boss (seemingly for entertainment purposes). These were removed.